N-Acetyl Epitalon Amidate
Preclinical ResearchN-Acetyl-Epitalon Amidate
A modified, more stable form of the pineal tetrapeptide Epitalon, researched for telomerase activity and circadian/pineal regulation.
Dose
0.5 mg - 1 mg
Route
Subcutaneous injection, Intranasal
Cycle
10-20 days
Storage
2°C to 8°C (refrigerator) away from light
What is N-Acetyl Epitalon Amidate?
N-Acetyl Epitalon Amidate is a synthetic tetrapeptide, a modified analog of Epitalon (Ala-Glu-Asp-Gly). Epitalon is derived from the synthetic peptide preparation Epithalamin, originally isolated from the bovine pineal gland. The N-terminal acetylation and C-terminal amidation modifications are hypothesized to improve the peptide's stability against enzymatic degradation and potentially enhance its bioavailability and half-life compared to the parent peptide. Research focuses on its potential role in modulating pineal gland function, regulating circadian rhythms, and influencing telomerase activity. The majority of studies on Epitalon and its analogs, including N-Acetyl Epitalon Amidate, have been conducted in animal models or in vitro settings, with some limited human observational studies on Epitalon itself. No FDA-approved indications exist for this compound.
Key Benefits
- Telomerase research
- Pineal and circadian regulation
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Molecular Information
Molecular weight
495.53 g/mol
Type
Synthetic tetrapeptide analog
Amino acid sequence
Ac-Ala-Glu-Asp-Gly-NH2
This is an N-terminally acetylated and C-terminally amidated analog of the native Epitalon (Ala-Glu-Asp-Gly). The modifications are designed to enhance stability and bioavailability.
Pharmacokinetics
Time to peak
15-30 minutes (subcutaneous, based on similar peptides)
Half-life
2 h
Time to clear
Within 4 hours (based on Epitalon's rapid metabolism)
Based on pharmacokinetic studies of Epitalon and general peptide pharmacokinetics. Specific data for N-Acetyl Epitalon Amidate are limited in peer-reviewed literature.
Research Indications
Telomerase Activity Modulation
Preclinical studies on Epitalon (the parent peptide) have indicated potential for modulating telomerase activity, an enzyme involved in maintaining telomere length. N-Acetyl Epitalon Amidate is hypothesized to share or enhance this activity. (Animal/In-vitro studies)
Antioxidant Defense
Some research suggests Epitalon may influence antioxidant enzyme systems, potentially reducing oxidative stress. Further research is needed to determine if N-Acetyl Epitalon Amidate exhibits similar properties. (Animal/In-vitro studies)
Research Protocols
| Goal | Dose | Frequency | Route |
|---|---|---|---|
| Investigation of general anti-aging markers and telomerase activity | 0.5 mg | Once daily | Subcutaneous injection |
| Exploration of circadian rhythm and sleep cycle modulation | 0.5 mg | Once daily (evening) | Subcutaneous injection |
| Maximum tolerated dose and initial efficacy screening | 1 mg | Once daily | Subcutaneous injection |
| Comparison of administration routes for bioavailability | 0.5 mg | Once daily | Intranasal (in solution) |
The typical dose and cycle lengths are derived from research protocols involving Epitalon, adjusted for the modified analog. Optimal timing (morning vs. evening) may be explored based on the desired research outcome (e.g., sleep regulation).
Peptide Interactions
- Monitor Combination
Melatonin
Epitalon and its analogs are thought to influence endogenous melatonin production. Combining with exogenous melatonin could potentially amplify or disrupt natural melatonin cycles, requiring careful observation in research.
- Monitor Combination
Other GHRH or GH secretagogues
Given the broad hormonal influences potentially associated with pineal peptides, combining with compounds affecting Growth Hormone (GH) axis may warrant careful observation in research protocols. Specific interactions are not well-studied.
- Monitor Combination
Immunomodulators
Some research on Epitalon suggests immunomodulatory effects. Combining with other immunomodulators could have additive or synergistic effects that require monitoring in a research setting. (Preclinical data on Epitalon)
- Monitor Combination
Thyroid hormones
There is some historical research suggesting a relationship between pineal peptides and thyroid function. Combining N-Acetyl Epitalon Amidate with thyroid hormone preparations would necessitate careful monitoring. (Preclinical data on Epitalon)
How to Reconstitute
- 1Gather the lyophilized peptide vial, a sterile syringe, and bacteriostatic water.
- 2Clean the rubber stopper of the peptide vial and the bacteriostatic water vial with alcohol swabs.
- 3Draw the desired amount of bacteriostatic water (e.g., 1 mL for a 5 mg vial to achieve 5 mg/mL concentration) into the sterile syringe.
- 4Slowly inject the bacteriostatic water into the peptide vial, directing it down the side of the vial to minimize direct contact with the powder.
- 5Remove the syringe and gently swirl the vial for several minutes until the powder is completely dissolved. Do not shake.
- 6Visually inspect the solution to ensure it is clear and free of particulate matter. If not, continue swirling gently.
Use bacteriostatic water for reconstitution to extend the shelf life of the reconstituted solution. Avoid vigorous shaking; gently swirl the vial to dissolve the powder completely.
What to Expect
- Lyophilized powder appears as a white, fluffy pellet at the bottom of the vial.
- After reconstitution, the solution should be clear and colorless.
- Local redness or slight discomfort at the injection site is possible but usually transient.
- Changes in subjective well-being or sleep patterns may be reported in anecdotal accounts, though robust human evidence for N-Acetyl Epitalon Amidate is lacking.
- No immediate dramatic effects are typically expected; any potential effects are often subtle and cumulative over a research cycle.
Side Effects & Safety
- This compound is for research use only and not for human consumption or therapeutic use. It is not an FDA-approved drug.
- Injectable peptides should always be administered using sterile techniques to prevent infection.
- Proper disposal of sharps (needles and syringes) is crucial to prevent accidental injury or contamination.
- The long-term safety profile of N-Acetyl Epitalon Amidate in humans is unknown, as research is primarily preclinical.
- Always store the peptide and reconstituted solution according to instructions to maintain stability and potency.
- Monitor for any signs of allergic reaction (e.g., rash, swelling, difficulty breathing) and discontinue use immediately if observed.
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