GLOW
Preclinical ResearchGLOW blend (GHK-Cu + BPC-157 + TB-500)
A three-peptide blend of GHK-Cu, BPC-157 and TB-500 sold in a single vial and researched for skin, hair and soft-tissue repair.
Dose
Blend dosing is set by the label ratio, not by a single number. A common research-market vial of 50 mg GHK-Cu / 10 mg BPC-157 / 10 mg TB-500 is typically dosed so that roughly 250-500 mcg BPC-157 and 250-500 mcg TB-500 are delivered per administration, which brings 1.25-2.5 mg GHK-Cu along with it.
Route
Subcutaneous injection, Localized subcutaneous injection
Cycle
4-8 weeks
Storage
Store the sealed lyophilized vial at 2-8°C protected from light. Long-term storage at -20°C preserves potency for 12-24 months. Copper peptide blends are light-sensitive, so keep the vial in its box.
What is GLOW?
GLOW is not a compound in its own right — it is a convenience blend of three separately studied peptides supplied premixed in one lyophilized vial. GHK-Cu is a copper-binding tripeptide with substantial in-vitro and topical human cosmetic data on collagen synthesis and skin remodeling. BPC-157 is a synthetic pentadecapeptide derived from a gastric protein, studied almost exclusively in rodents for tendon, ligament, muscle and gut healing. TB-500 is the synthetic active fragment of thymosin beta-4, studied in animals for actin regulation, cell migration and angiogenesis during tissue repair. Vendors vary widely in the ratio they use; the most common research-market ratio is 50 mg GHK-Cu / 10 mg BPC-157 / 10 mg TB-500 per vial, but 30/10/10 and 50/5/5 also circulate, so the label on the vial is the only reliable guide to what a given unit of volume actually delivers. There is no published study of the blend itself: everything known about it is inferred from the three components studied separately, and nothing in the literature establishes that combining them is additive, synergistic, or safe over long periods.
Key Benefits
- Researched for soft-tissue and tendon repair
- Researched for collagen and elastin synthesis
- Researched for wound closure and angiogenesis
- Researched for hair follicle support
- Convenience of three repair-oriented peptides in one vial
Mechanism of Action
- GHK-Cu: copper delivery to fibroblasts, upregulation of collagen, elastin and glycosaminoglycan synthesis
- BPC-157: upregulation of VEGFR2 signaling and growth factor receptor expression in injured tissue (rodent data)
- TB-500: actin sequestration promoting cell migration, angiogenesis and reduced fibrosis (animal data)
Best For
- Soft-tissue recovery research
- Skin and cosmetic research
- Post-injury repair research
Body Systems
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Molecular Information
Molecular weight
No single value — blend. GHK-Cu 340.7 g/mol (copper complex) · BPC-157 1419.5 g/mol · TB-500 ~4963 g/mol
Length
3 aa (GHK-Cu) · 15 aa (BPC-157) · synthetic thymosin beta-4 fragment (TB-500)
Type
Multi-compound blend (three-part vial)
Amino acid sequence
GHK-Cu: Gly-His-Lys + Cu(II) | BPC-157: Gly-Glu-Pro-Pro-Pro-Ala-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val | TB-500: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Lys-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH
GLOW is a three-peptide blend, so it has no single molecular weight, sequence or amino acid count. What a given unit of volume delivers depends on the mg ratio printed on your vial label, which differs between vendors. Calculate from the one component you are targeting, not the combined vial weight.
Pharmacokinetics
Time to peak
Per component: GHK-Cu peaks within minutes of subcutaneous injection; BPC-157 around 1-2 hours; TB-500 distributes over hours.
Half-life
48 h
Time to clear
Staggered. GHK-Cu clears within minutes to about an hour, BPC-157 within roughly 1-2 days, and TB-500 over several days. The listed half-life reflects TB-500, the longest-acting component.
No pharmacokinetic study has been run on the GLOW blend. Every figure here is inferred from the three components studied separately, largely in animal models.
Research Indications
BPC-157 rodent models
Faster tendon-to-bone and muscle healing reported in rats. No controlled human trial exists.
TB-500 cell migration
Actin sequestration supports cell migration and angiogenesis during animal wound repair.
Research Protocols
| Goal | Dose | Frequency | Route |
|---|---|---|---|
| Soft-tissue recovery research | 250-500 mcg BPC-157 equivalent | Once daily, 4-8 weeks | Subcutaneous, abdominal |
| Skin and cosmetic research | 1.25-2.5 mg GHK-Cu equivalent at a typical ratio | Once daily | Subcutaneous, rotating sites |
| Localized / site-directed research | 250 mcg BPC-157 equivalent | Once daily for 2-4 weeks | Subcutaneous near the target tissue |
| TB-500-weighted loading | 2-2.5 mg TB-500 equivalent per week, split | 2-3x weekly for 4 weeks | Subcutaneous |
| Maintenance after an initial block | Half the loading amount | 2-3x weekly | Subcutaneous, after a 4-week break |
No pharmacokinetic reason to prefer a particular time of day. Consistency matters more than clock time; many protocols use evening dosing simply because it is easier to keep to.
Peptide Interactions
How to Reconstitute
- 1Let the sealed vial come to room temperature before you touch it.
- 2Wipe both the blend vial and the bacteriostatic water stopper with a fresh alcohol swab.
- 3Draw your chosen volume of bacteriostatic water — 2 mL and 3 mL are the common choices for a 70 mg blend vial.
- 4Angle the needle so the water runs down the inside wall of the vial rather than jetting onto the powder.
- 5Swirl gently until the powder dissolves. Never shake: the copper component makes the solution look dramatic when agitated, but the peptides are still fragile.
- 6Label the vial with the date and the mg of each component, then refrigerate.
Because the vial holds three peptides at different strengths, your calculator needs the mg of the component you are actually targeting — usually BPC-157 or TB-500 — not the combined vial weight. Work out the volume for that component and accept whatever amount of the other two comes along with it.
What to Expect
- A blue-green solution after reconstitution — that is the copper component and is expected.
- Stinging or flushing at the injection site, most often from GHK-Cu. Rotate sites.
- No way to attribute any change to a specific component — three variables, one syringe.
- Soft-tissue changes described over 2-6 weeks in animal work, not days.
- Skin quality changes described over 4-12 weeks, and only in topical GHK-Cu studies.
- Vendor-to-vendor variation in what a given volume delivers, because ratios differ.
Side Effects & Safety
| Effect | Frequency | Severity |
|---|---|---|
| Injection site redness, flushing or stinging | Common | Mild |
| Injection site irritation from the copper component | Common | Mild |
| Lightheadedness or fatigue after dosing | Uncommon | Mild |
| Headache | Uncommon | Mild |
| Unknown long-term effects from combined chronic exposure | Unknown | Unknown |
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Open CalculatorPeptides discussed here are for research purposes only. Nothing on this page is medical advice. Always consult a qualified professional before making health decisions.