AHK-Cu
Preclinical ResearchAHK-Cu (Alanine-Histidine-Lysine copper peptide)
A copper-binding tripeptide (Ala-His-Lys) studied primarily in topical cosmetic research for hair follicle stimulation and dermal support.
Dose
Topical application based on formulation concentration
Route
Topical
Cycle
Typically several weeks to months in research studies
Storage
2°C to 8°C (refrigerated) in a sealed, dark, airtight container, away from light and moisture
What is AHK-Cu?
AHK-Cu, or Alanine-Histidine-Lysine-Copper, is a synthetic tripeptide that forms a stable complex with copper ions. It is structurally similar to GHK-Cu but has an alanine residue in place of glycine. Research on AHK-Cu primarily focuses on its potential dermatological and cosmetic applications, particularly for hair growth stimulation and skin health. Studies, mostly in vitro and animal models, investigate its role in promoting collagen and elastin synthesis, antioxidant activity, and angiogenesis, which are beneficial for wound healing, anti-aging, and hair follicle vitality. As a research chemical, it is typically explored in topical formulations.
Key Benefits
- Hair follicle research
- Dermal blood vessel support
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Molecular Information
Molecular weight
426.96 g/mol (for AHK-Cu complex with one copper ion)
Length
3 amino acids
Type
Peptide-copper complex
Amino acid sequence
Ala-His-Lys (AHK)
AHK-Cu is a tripeptide (Alanine-Histidine-Lysine) that forms a complex with a copper ion.
Research Indications
Hair Follicle Stimulation
Animal studies (e.g., mouse models) have shown that topical application of AHK-Cu can increase hair follicle size and stimulate the anagen phase of the hair cycle. Evidence is from animal/in-vitro only.
Prevention of Hair Loss
Preclinical investigations suggest AHK-Cu may help in reducing hair loss by supporting hair follicle health and prolonging the anagen phase. Evidence is from animal/in-vitro only.
Research Protocols
| Goal | Dose | Frequency | Route |
|---|---|---|---|
| Hair Growth Stimulation (Topical) | 0.1% to 1% AHK-Cu in a carrier solution/cream | Once daily | Topical application to shaved skin area (e.g., mouse dorsal skin) |
| Dermal Fibroblast Proliferation (In Vitro) | 1-10 µM AHK-Cu in cell culture media | Single or multiple applications over 24-72 hours | Direct addition to cell culture |
| Wound Healing Acceleration (Topical) | 0.5% to 2% AHK-Cu in a gel or cream base | Once or twice daily | Topical application to experimental wound sites (e.g., full-thickness excisional wounds in rats) |
| Collagen Synthesis Promotion (In Vitro) | 5-15 µM AHK-Cu in cell culture media | Continuous exposure for 48-72 hours | Direct addition to cell culture of fibroblasts |
| Angiogenesis Research (In Vitro/In Vivo) | Varies (e.g., 1-10 µM in vitro, 0.1-1% topical in vivo) | Single exposure for in vitro; daily for in vivo | Cell culture or topical application |
Timing for topical application varies significantly based on specific research objectives and formulation. Consistent daily application is often used in studies evaluating long-term effects.
Peptide Interactions
- Avoid
Strong Acids/Bases
Extreme pH can degrade the peptide structure and/or destabilize the copper complex, leading to loss of activity.
- Use Caution
Chelating Agents (e.g., EDTA, Citric Acid)
Strong chelating agents may compete with the peptide for copper binding, potentially disrupting the AHK-Cu complex and reducing its efficacy. Monitor for desired effects.
- Use Caution
Oxidizing Agents (e.g., Hydrogen Peroxide)
Can potentially oxidize the peptide or copper, leading to degradation or altered activity. Formulate carefully.
- Use Caution
Reducing Agents
May reduce the copper ion, potentially altering the stability and biological activity of the complex.
- Monitor Combination
Other Peptides or Growth Factors
While often used in combination in cosmetic research, specific interactions or synergistic/antagonistic effects with other bioactive peptides or growth factors should be carefully evaluated in a research setting.
How to Reconstitute
- 1Verify the specific solvent recommended by the AHK-Cu supplier for topical formulation or solution preparation.
- 2Using sterile technique, carefully remove the cap from the lyophilized AHK-Cu vial.
- 3Using a sterile syringe, draw up the required volume of the solvent.
- 4Slowly inject the solvent into the AHK-Cu vial, directing the flow down the side of the glass to avoid direct impact on the powder.
- 5Gently swirl (do not shake vigorously) the vial to allow the AHK-Cu powder to fully dissolve. This may take a few minutes.
- 6Once completely dissolved and clear, the AHK-Cu solution is ready for incorporation into a research formulation or direct topical application according to the protocol.
AHK-Cu is predominantly used in topical formulations, not typically reconstituted for injection. If supplied as lyophilized powder for research into solution-based topical application, it would be dissolved in an appropriate solvent (e.g., deionized water, saline, or specific formulation bases) at the desired concentration.
What to Expect
- In vitro studies show AHK-Cu can promote cell proliferation and collagen synthesis in dermal fibroblasts.
- Animal studies suggest potential for AHK-Cu to stimulate hair growth and follicle size.
- Preclinical research indicates AHK-Cu may exhibit antioxidant properties beneficial for skin.
- Topical application in animal models has shown potential to improve wound healing rates.
- Research points to AHK-Cu's role in promoting angiogenesis, which is important for tissue regeneration.
Side Effects & Safety
- AHK-Cu is for research purposes only and not for human consumption or medical use.
- Handle with appropriate personal protective equipment (gloves, eye protection) in a laboratory setting.
- Avoid direct contact with skin and eyes; in case of contact, rinse thoroughly with water.
- Do not inhale powder or vapors if handling raw material.
- Dispose of unused material and waste in accordance with local regulations for research chemicals.
- Research on potential irritancy or sensitization with prolonged topical use in humans is limited.
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Open CalculatorPeptides discussed here are for research purposes only. Nothing on this page is medical advice. Always consult a qualified professional before making health decisions.